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-80°C
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英文名：
PARPtrap™ Assay Kit for PARP1
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数量：
1
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供应商：
BPS Bioscience Inc.
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CAS号：
/
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规格：
盒
The PARPtrap™ Assay Kit for PARP1 is designed to measure PARP1/某~某 complex formation in a high throughput screening assay using fluorescence polarization (FP). PARP1 is known to bind damaged 某~某 through its 某~某-binding domains. Binding to 某~某 activates PARP1 and in the presence of NAD+ PARP1 ribosylates itself (auto-ribosylation), what in consequence leads to PARP1 dissociation from the 某~某 due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, PARP remains bound to the 某~某, a phenomenon termed trapping. Trapped PARP-某~某 complexes h某~某e been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment.The key to the PARPtrap™ Assay Kit for PARP1 is the fluorescent-labeled oligonucleotide duplex. In the absence of ribosylation, PARP1 binds to the fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after auto-ribosylation, PARP1 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP1 inhibitor results in trapping of PARP1 to the fluorescent oligonucleotide duplex, and increases the FP signal in a dose dependent manner.The PARPtrap™ Assay Kit for PARP1 is a fluorescence polarization homogeneous assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
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http://www.bpsbioscience.com.cn/Products-37923810.html
https://www.chem17.com/st542058/product_37923810.html
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